ISSN: 1300-7777 E-ISSN: 1308-5263
Influence of L-carnitine on Stored Rat Blood: A study on Plasma [Turk J Hematol]
Turk J Hematol. Ahead of Print: TJH-82698 | DOI: 10.4274/tjh.2016.0343  

Influence of L-carnitine on Stored Rat Blood: A study on Plasma

Carl Hsieh, Vani Rajashekharaiah
Department of Biotechnology, Center for Post Graduate Studies, Jain University, Bangalore

Objective: Plasma acts as a good indicator of oxidative stress in blood. L-carnitine is an antioxidant that reduces metabolic stress in cells, thereby providing a protective effect against oxidative stress. L-carnitine as an additive in storage has not been explored. Thus, this study attempts to analyze the role of L-carnitine in blood storage solution, Citrate Phosphate Dextrose Adenine (CPDA)-1 through oxidative stress (OS) markers - antioxidant enzymes, lipid peroxidation and protein oxidation.
Materials and Methods: Blood was collected from male Wistar rats and stored in CPDA-1 solution with L-carnitine (10 mM, 30 mM and 60 mM) and without L-carnitine (controls). Plasma was isolated every 5th day and the OS markers were analyzed.
Results: SOD and SH increased over storage in controls, LC 30 and LC 60. CAT increased in LC 30 and LC 60 during storage. While TBARS and PrC in all groups increased initially and reduced towards the end of storage. SOD and SH were maintained, while TBARS and PrC increased in LC 10.
Conclusion: L-carnitine was beneficial in terms of increased antioxidant capacity and sulfhydryls and decreased lipid peroxidation. Thus, it forms the basis for further studies on L-carnitine as a constituent in storage solutions.

Keywords: L-carnitine, plasma, antioxidant enzymes, lipid peroxidation, protein oxidation.




Corresponding Author: Vani Rajashekharaiah, India


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