ISSN: 1300-7777 E-ISSN: 1308-5263
The Effect of Bone Marrow Mesenchymal Stem Cells on the Granulocytic Differentiation of HL-60 Cells [Turk J Hematol]
Turk J Hematol. Ahead of Print: TJH-38039 | DOI: 10.4274/tjh.2016.0498  

The Effect of Bone Marrow Mesenchymal Stem Cells on the Granulocytic Differentiation of HL-60 Cells

Hossein Nikkhah1, Elham Safarzadeh2, Karim Shamsasenjan1, Mehdi Yousefi2, Parisa Lotfinejad3, Mehdi Talebi1, Mozhde Mohammadian4, Farhoud Golafshan5, Ali Akbar Movassaghpour1
1Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2Drug Applied Research Center, Tabriz University of Medical Science, Tabriz, Iran, Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran
3Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran, Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran
4Amol faculty of paramedical sciences, Mazandaran university of medical sciences, Sari, Iran
5Department of Biology, Hamline University, Saint Paul, MN 55104, USA

The mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into a variety of cell types. They control the process of haematopoiesis by secreting regulatory cytokines and growth factors and by the expression of important cell adhesion molecules for cell-to-cell interactions. This investigation intended to examine the effect of bone marrow-derived mesenchymal stem cells (BM-MSCs) on the differentiation of the HL-60 cells according to morphological evaluation, flow cytometry analysis, andgene expression profile. Materials and Methods: The BM-MSCs were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% Foetal bovine serum (FBS). After the third passage, the BM-MSCs were irradiated by 30 Gray. To compare how the HL-60 cells differentiate in the groups treated differently, the HL-60 cells were cultured in RPMI-1640 and supplemented with 10% FBS. The HL-60 cells were seeded into six-well culture plates and treated with All Trans Retinoic Acid (ATRA), BM-MSCs, BM-MSCs in combination with ATRA, while one well remained as untreated HL-60 cells. The expression levels of the granulocyte subset-specific genes in the HL-60 cells were assayed by real-time PCR (RT-PCR). Results: Our results revealed that the BM-MSCs support the granulocytic differentiation of the human promyelocytic leukaemia cell line, HL-60. Conclusion: Based on the results of this study, we concluded that the BM-MSCs may be an effective resource in reducing or even preventing ATRA’s side effects and may promote differentiation for short medication periods. Though the BM-MSCs are effective resources, more complementary studies are necessary to improve this differentiation mechanism in clinical cases.

Keywords: Mesenchymal stem cells, HL-60 cells, Differentiation, ATRA




Corresponding Author: Ali Akbar Movassaghpour, Iran


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