ISSN: 1300-7777 E-ISSN: 1308-5263
Antioxidants attenuate oxidative stress-induced hidden blood loss in rats [Turk J Hematol]
Turk J Hematol. Ahead of Print: TJH-25633 | DOI: 10.4274/tjh.2016.0469  

Antioxidants attenuate oxidative stress-induced hidden blood loss in rats

Hong Qian1, Tao Yuan2, Jian Tong3, Wen Shuang Sun1, Jiajia Jin4, Wen Xiang Chen5, Jia Meng2, Nirong Bao2, Jianning Zhao2
1Department of Orthopedics, Nanjing General Hospital Of Nanjing Military Command, Southeast University
2Department of Orthopedic, Jinling Hospital, School of Medical, Nanjing University
3Department of Orthopedics,School of Clinical Medicine,Nanjing University/Nanjing General Hospital of Nanjing Military Command. Nanjing
4Department of Respiratory Medicine, Nanjing General Hospital Of Nanjing Military Command, Southeast University
5Southern Medical University, Department of Orthopedics of Jinling Hospital(Nanjing),School of Medicine

Hidden blood loss (HBL), commonly seen post total knee or hip arthroplasty, causes postoperative anemia even after reinfusion or blood transfusion based on the visible blood loss volume. Recent studies demonstrated oxidative stress might be involved in HBL. However, whether antioxidants proanthocyanidin (PA) or hydrogen water (HW) can ameliorate HBL remains poorly understood. The aim of the study was to evaluate the effect of PA and HW on HBL. Rat HBL model was established through administration of linoleic acid with or without treatment of PA or HW. The levels of hemoglobin, red blood cell count, superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-PX) activity, Malondialdehyde (MDA), and ferryl hemoglobin (Hb) were measured. RBC and Hb values as well as the activity of SOD and GSH-PX were reduced after administration of linoleic acid, which were ameliorated by treatment with PA or HW. In addition, the quantity of MDA was significantly decreased along with the administration of PA or HW. In conclusion, PA or HW could ameliorate HBL in rat model through reducing oxidative stress, suggesting they might be used as novel therapeutic approach in the prophylaxis or treatment of HBL in clinic.





Corresponding Author: Jianning Zhao, China


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