Antioxidants Attenuate Oxidative Stress-Induced Hidden Blood Loss in RatsHong Qian1, Tao Yuan2, Jian Tong3, Wen Shuang Sun1, Jiajia Jin4, Wen Xiang Chen5, Jia Meng2, Nirong Bao2, Jianning Zhao21Southeast University Nanjing General Hospital of Nanjing Military Command, Clinic of Orthopedics, Nanjing, China
2Nanjing University Faculty of Medicine, Jinling Hospital, Clinic of Orthopedics, Nanjing, China
3Nanjing University Faculty of Medicine, Nanjing General Hospital of Nanjing Military Command, Clinic of Orthopedics, Nanjing, China
4Southeast University Nanjing General Hospital of Nanjing Military Command, Department of Respiratory Medicine, Nanjing, China
5Southern Medical University Faculty of Medicine, Department of Orthopedics, Nanjing, China
Objective: Hidden blood loss (HBL), commonly seen after total knee or hip arthroplasty, causes postoperative anemia even after reinfusion or blood transfusion based on the visible blood loss volume. Recent studies demonstrated that oxidative stress might be involved in HBL. However, whether the antioxidants proanthocyanidin (PA) or hydrogen water (HW) can ameliorate HBL remains poorly understood. The aim of this study was to evaluate the effects of PA and HW on HBL.
Materials and Methods: A rat HBL model was established through administration of linoleic acid with or without treatment with PA or HW. The levels of hemoglobin (Hb), red blood cell (RBC) count, superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-PX) activity, malondialdehyde (MDA), and ferryl Hb were measured.
Results: RBC and Hb values as well as the activity of SOD and GSHPX were reduced after administration of linoleic acid, which was ameliorated by treatment with PA or HW. In addition, the quantity of MDA was significantly decreased with the administration of PA or HW.
Conclusion: PA and HW could ameliorate HBL in a rat model by reducing oxidative stress, suggesting that they might be used as a novel therapeutic approach in the prophylaxis or treatment of HBL in clinics.
Keywords: Hidden blood loss, Antioxidants, Proanthocyanidin, Hydrogen water
Antioksidanlar Sıçanlarda Oksidatif Stres ile Oluşan Gizli Kan Kaybını ZayıflatırHong Qian1, Tao Yuan2, Jian Tong3, Wen Shuang Sun1, Jiajia Jin4, Wen Xiang Chen5, Jia Meng2, Nirong Bao2, Jianning Zhao21Southeast University Nanjing General Hospital of Nanjing Military Command, Clinic of Orthopedics, Nanjing, China
2Nanjing University Faculty of Medicine, Jinling Hospital, Clinic of Orthopedics, Nanjing, China
3Nanjing University Faculty of Medicine, Nanjing General Hospital of Nanjing Military Command, Clinic of Orthopedics, Nanjing, China
4Southeast University Nanjing General Hospital of Nanjing Military Command, Department of Respiratory Medicine, Nanjing, China
5Southern Medical University Faculty of Medicine, Department of Orthopedics, Nanjing, China
Amaç: Total diz veya kalça artroplastisinden sonra yaygın olarak görülen gizli kan kaybı (GKK), görülebilir kan hacmi kaybına dayanan reinfüzyon veya kan nakli sonrasında bile postoperatif anemiye neden olur. Son yıllarda yapılan çalışmalar oksidatif stresin GKK’yla ilişkili olabileceğini göstermiştir. Bununla birlikte, proantosiyanidin (PA) veya hidrojenli su (HS) antioksidanlarının GKK’yi iyileştirip iyileştirmediği anlaşılamamıştır. Bu çalışmanın amacı PA ve HS’nin GKK üzerindeki etkilerini değerlendirmektir.
Gereç ve Yöntem: PA veya HS ile muamele edilmiş veya edilmemiş olan linoleik asit uygulaması ile bir sıçan GKK modeli oluşturulmuştur. Hemoglobin (Hb), kırmızı kan hücresi sayısı (RBC), süperoksit dismutaz (SOD) aktivitesi, glutatyon peroksidaz (GSH-PX) aktivitesi, malondialdehit (MDA) ve ferril Hb düzeyleri ölçüldü.
Bulgular: PA veya HS verilen linoleik asit uygulaması ile RBC ve Hb değerlerinin yanı sıra SOD ve GSH-PX aktiviteleri azaltıldı. Buna ek olarak, PA veya HS uygulaması ile MDA miktarı önemli ölçüde azaldı.
Sonuç: PA ve HS bir sıçan modelinde oksidatif stresi azaltarak GKK’yi düzeltebildiğinden, kliniklerdeki GKK profilaksisi veya tedavisinde yeni bir terapötik yaklaşım olarak kullanılabileceklerini düşündürmektedir.
Anahtar Kelimeler: Gizli kan kaybı, Antioksidanlar, Proantosiyanidin, Hidrojenli su
Hong Qian, Tao Yuan, Jian Tong, Wen Shuang Sun, Jiajia Jin, Wen Xiang Chen, Jia Meng, Nirong Bao, Jianning Zhao. Antioxidants Attenuate Oxidative Stress-Induced Hidden Blood Loss in Rats. Turk J Hematol. 2017; 34(4): 334-339
Corresponding Author: Jianning Zhao, China |
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