ISSN: 1300-7777 E-ISSN: 1308-5263
Evaluation of neutrophil functions in obstructive jaundice patients [Turk J Hematol]
Turk J Hematol. 2004; 21(4): 189-196

Evaluation of neutrophil functions in obstructive jaundice patients

Geetha Arumugam1, Surendran Rajagopal2
1Bharathi Womens College, Biochemistry, Chennai Tamil Nadu, Indıa
2Stanely Medical College Hospital, Gastroenterology, Chennai Tamil Nadu, Indıa

Jaundiced surgical patients have a high incidence of post-operative complications and sepsis is a major factor in the high mortality and morbidity of obstructive jaundice patients after surgery. Cachexia and immune suppression have been shown to be involved in this post-operative complications. Since neutrophils dysfunction is recognized in recurrent infections in general, the aim of the study was to evaluate the functional status of neutrophils in jaundiced patients. Phagocytosis, bactericidal activity, mitogenic stimulation of T-lymphocytes and myeloperoxidase activity were measured in the neutrophils isolated from obstructive jaundice patients. Phagocytic and bactericidal activities of neutrophils were significantly low in obstructive jaundice patients. The number of cells stained for myeloperoxidase was significantly low in obstructive jaundice patients. There was a high level of stimulation and proliferation exhibited by lymphocytes when challenged by conconavalin-A and lipopolysaccharide in vitro. The functional impairment of neutrophils may be responsible for the immunity alteration in obstructive jaundice patients and this may be responsible for the post-operative complications in obstructive jaundiced patients.

Keywords: Obstructive jaundice, Phagocytosis, Bactericidal activity, T-cells, stimulation, Myeloperoxidase.


Tıkanma sarılıklı hastalarda nötrofil ifllevleri

Geetha Arumugam1, Surendran Rajagopal2
1Bharathi Womens College, Biochemistry, Chennai Tamil Nadu, Indıa
2Stanely Medical College Hospital, Gastroenterology, Chennai Tamil Nadu, Indıa

Sarılıklı cerrahi hastaları sık cerrahi sonrası komplikasyonlarla karşılaşırlar ve sepsis, tıkanma sarılıklı hastalarda cerrahi sonrası mortalite ve morbiditede ana faktördür. Bu cerrahi sonrası komplikasyonlarda kafleksi ve immünsüpresyonda rol oynar. Tekrarlayan infeksiyonlarda nötrofil disfonksiyonu gözlendiğinden, bu çalışmanın amacı sarılıklı hastalarda nötrofil işlevlerine bakılmasıdır. Bunun için sarılıklı hastalardan elde edilen nötrofillerde fagositoz, bakterisidal aktivite, T-lenfositlerin mitojenik uyarımı ve miyeloperoksidaz aktivitesi ölçülmüştür. Sonuçta tıkanma sarılıklı hastalarda nötrofillerin fagositik ve bakterisidal aktiviteleri belirgin derecede düşük bulunmuştur. Tıkanma sarılıklı hastalarda miyeloperoksidaz alan hücrelerin sayısında da düşüklük görülmüştür. in vitro konkonavalin-A ve lipopolisakkarit ile karşılaşan lenfositlerde de yüksek düzeyli stimülasyon ve proliferasyon görülmüfltür. Nötrofillerin işlevsel bozuklukları tıkanma sarılıklı hastaların immünitelerinde bozulmadan sorumlu olabilir ve bu da cerrahi sonrası komplikasyonları arttırabilir.

Anahtar Kelimeler: Tıkanma sarılığı, Fagositoz, Bakterisidal etki, T-hücre, uyarımı, Miyeloperoksidaz.


Geetha Arumugam, Surendran Rajagopal. Evaluation of neutrophil functions in obstructive jaundice patients. Turk J Hematol. 2004; 21(4): 189-196

Corresponding Author: Geetha Arumugam, India


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