ISSN: 1300-7777 E-ISSN: 1308-5263
Antibacterial Activities of Ankaferd Hemostat (ABS) on Shiga Toxin-Producing Escherichia coli and Other Pathogens Significant in Foodborne Diseases [Turk J Hematol]
Turk J Hematol. 2017; 34(1): 93-98 | DOI: 10.4274/tjh.2015.0073  

Antibacterial Activities of Ankaferd Hemostat (ABS) on Shiga Toxin-Producing Escherichia coli and Other Pathogens Significant in Foodborne Diseases

Ahmet Koluman1, Nejat Akar2, İbrahim C. Haznedaroğlu3
1Republic of Turkey Ministry of Food, National Food Reference Laboratory, Department of Mineral Analyses, Agriculture, and Livestock, Ankara, Turkey
2TOBB-ETU Hospital, Clinic of Pediatric Hematology, Ankara, Turkey
3Hacettepe University Faculty of Medicine, Department of Adult Hematology, Ankara, Turkey

Objective: Ankaferd hemostat (Ankaferd Blood Stopper®, ABS)- induced pharmacological modulation of essential erythroid proteins can cause vital erythroid aggregation via acting on fibrinogen gamma. Topical endoscopic ABS application is effective in the controlling of gastrointestinal (GI) system hemorrhages and/or infected GI wounds. Escherichia coli O157: H7, the predominant serotype of enterohemorrhagic E. coli, is a cause of both outbreaks and sporadic cases of hemorrhagic colitis. The aim of this study is to examine the effects of ABS on 6 different Shiga toxigenic E. coli serotypes including O26, O103, O104, O111, O145, and O157 and on other pathogens significant in foodborne diseases, such as Salmonella Typhimurium, Campylobacter jejuni, and Listeria monocytogenes, were also assessed.
Materials and Methods: All strains were applied with different amounts of ABS and antimicrobial effect was screened. S. Typhimurium groups were screened for survival using the fluorescence in situ hybridization technique.
Results: The relative efficacy of ABS solutions to achieve significant logarithmic reduction in foodborne pathogens E. coli O157: H7 and non-O157 serogroups and other emerging foodborne pathogens is demonstrated in this study. ABS has antibacterial effects.
Conclusion: Our present study indicated for the first time that ABS may act against E. coli O157: H7, which is a cause of thrombotic thrombocytopenic purpura, hemolytic-uremic syndrome, and hemorrhagic colitis. The interrelationships between colitis, infection, and hemostasis within the context of ABS application should be further investigated in future studies.

Keywords: Ankaferd Blood Stopper, Shiga-toxigenic Escherichia coli, Salmonella, Campylobacter, Listeria monocytogenes


Ankaferd Hemostat’ın (ABS) Shiga Toksijenik Escherichia coli ve Diğer Gıda Patojenleri Üzerine Antibakteriyel Etkisi

Ahmet Koluman1, Nejat Akar2, İbrahim C. Haznedaroğlu3
1Republic of Turkey Ministry of Food, National Food Reference Laboratory, Department of Mineral Analyses, Agriculture, and Livestock, Ankara, Turkey
2TOBB-ETU Hospital, Clinic of Pediatric Hematology, Ankara, Turkey
3Hacettepe University Faculty of Medicine, Department of Adult Hematology, Ankara, Turkey

Amaç: Ankaferd hemostat (Ankaferd Blood Stopper®, ABS) gamma fibrinojene etki ederek eritroid agregasyonuna neden olan farmakolojik modülasyondur. Topikal endoskopik ABS uygulaması gastrointestinal (Gİ) kanamalarda ve enfekte Gİ yaralarında etkili olmaktadır. Escherichia coli O157: H7, en sık karşılaşılan enterohemorajik Escherichia coli tipi olup sporadik veya salgınlar şeklinde hemorajik kolitin önemli bir etkenidir. Bu çalışmanın amacı ABS ile 6 farklı Shiga Toksijenik Escherichia coli serotipi (O26, O103, O104, O111, O145 ve O157) ve diğer önemli gıda kaynaklı patojenlerden Salmonella, Campylobacter ve Listeria monocytogenes üzerine etkisi değerlendirilmiştir.
Gereç ve Yöntem: Tüm patojenler hazırlanarak ABS’nin farklı miktarları uygulanmış ve antimikrobiyel etki izlenmiştir. Salmonella canlılığı floresan in situ hibridizasyon tekniği ile izlenmiştir.
Bulgular: ABS uygulamalarının sadece Escherichia coli O157 ve non- O157’ler üzerine değil aynı zamanda diğer patojenlerde de logaritmik azalma tetiklediği izlenmiştir. Bu çalışmada ABS ile farklı patojenler üzerine antibakteriyel etki gözlemlenmiştir.
Sonuç: Bu çalışma özellikle trombositopenik purpura, hemolitik üremik sendrom ve hemorajik kolit yönünden önemli Escherichia coli O157: H7’nin üzerine ABS’nin antimikrobiyel etkisi olduğunu belirleyen ilk çalışmadır. ABS uygulamalarının kolitis, enfeksiyon ve hemostaz ilişkisi daha ileri seviyede araştırılmalıdır.

Anahtar Kelimeler: Ankaferd Blood Stopper, Shigatoksijenik Escherichia coli, Salmonella, Campylobacter, Listeria monocytogenes


Ahmet Koluman, Nejat Akar, İbrahim C. Haznedaroğlu. Antibacterial Activities of Ankaferd Hemostat (ABS) on Shiga Toxin-Producing Escherichia coli and Other Pathogens Significant in Foodborne Diseases. Turk J Hematol. 2017; 34(1): 93-98

Corresponding Author: Ahmet Koluman, Türkiye


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