ISSN: 1300-7777 E-ISSN: 1308-5263
Critical Role of Sensitized Serum in Rejection of Allogeneic Bone Marrow Cells [Turk J Hematol]
Turk J Hematol. 2014; 31(3): 266-271 | DOI: 10.4274/Tjh.2012.0213  

Critical Role of Sensitized Serum in Rejection of Allogeneic Bone Marrow Cells

Lu Hong Xu, Jian Pei Fang, Wen Jun Weng, Hong Gui Xu
Sun Yat-sen University, Sun Yat-sen Memorial Hospital, Department Of Pediatrics, Guangzhou, China; Key Laboratory Of Malignant Tumor Gene Regulation And Target, Guangzhou, China

OBJECTIVE: Humoral immunity has been clearly implicated in solid organ transplantation, but little is known about the relationship between humoral immunity and hematopoietic stem cell transplantation. This study was designed to investigate that relationship.
METHODS: Sensitized serum was obtained from a sensitized murine model established by allogeneic
splenocyte transfusion. Sensitized serum was incubated with allogeneic bone marrow cells (BMCs) in vitro and the cytotoxicity was evaluated by the complement-dependent cytotoxicity method. Mice were transplanted with allogeneic BMCs incubated with sensitized serum after lethal irradiation. The engraftment was assayed by hematopoietic recovery and chimera analysis. Moreover, mice received passive transfer of sensitized serum 1 day prior to transplantation. Mortality was scored daily after bone marrow transplantation.
RESULTS: The in vitro experiments showed that sensitized serum was capable of impairing allogeneic BMCs through the complement-dependent cytotoxicity pathway. The animal studies showed that BMCs incubated with sensitized serum failed to rescue mice from lethal irradiation. The engraftment assay showed that the allogeneic BMCs incubated with sensitized serum were rejected with time in the recipients. Furthermore, the mice died of marrow graft rejection by transfer of sensitized serum prior to transplantation.
CONCLUSION: Taken together, our results indicated that sensitized serum played a critical role in graft rejection during hematopoietic stem cell transplantation.

Keywords: Sensitized serum, Bone marrow cells, Rejection, Transplantation


Sensitize Serumun Allojeneik Kök Hücrelerin Rejeksiyonunda Kritik Rolü

Lu Hong Xu, Jian Pei Fang, Wen Jun Weng, Hong Gui Xu
Sun Yat-sen University, Sun Yat-sen Memorial Hospital, Department Of Pediatrics, Guangzhou, China; Key Laboratory Of Malignant Tumor Gene Regulation And Target, Guangzhou, China

AMAÇ: Solid organ transplantasyonlarında humoral immunitenin önemi ile ilgili bilgilerimiz daha açık olmakla birlikte, humoral immunite ve hematopoetik kök hücre nakli arasındaki ilişki konusunda bilgilerimiz sınırlıdır. Bu çalışmada bu ilişkinin araştırılması hedeflenmiştir.
YÖNTEMLER: Sensitize bir fare modelinden allojeneik splenosit transfüzyonu elde edildi. Sensitize serum, allojeneik kemik iliği hücreleri ile in vitro olarak inkübe edildi ve sitotoksisite komplemana bağımlı sitotoksisite metodu ile değerlendirildi. İrradiasyon sonrası farelere sensitize serumda inkübe edilmiş allojeneik kemik iliği hücreleri transplante edildi. Engraftman, hematopoetik düzelme ve kimerizm analizleri ile değerlendirildi. Ayrıca, farelere transplanttan 1 gün önce sensitize serum pasif olarak uygulandı. Kemik iliği transplantasyonu sonrası mortalite günlük olarak skorlandı.
BULGULAR: İn vitro çalışmalar göstermiştir ki, sensitize serum komplemana bağımlı sitotoksisite üzerinden allojeneik kemik iliği hücrelerini etkilemektedir. Sensitize serum ile inkübe edilmiş kemik iliği hücreleri fareleri letal dozda irradiasyondan korumakta başarısız olmuştur. Sensitize serum ile inkübe edilen kemik iliği hücreleri alıcılarda zaman içinde rejeke edilmişlerdir. Ayrıca, transplantasyondan önce sensitize serum verilen fareler graft rejeksiyonu nedeniyle ölmüşlerdir.
SONUÇ: Bu bilgiler ışığında, sensitize serumun hematopoetik kök hücre transplantasyonunda oluşabilecek graft rejeksiyonunda kritik bir role sahip olduğu söylenebilir.

Anahtar Kelimeler: Sensitize serum, Kemik iliği hücreleri, Rejeksiyon, Transplantasyon


Lu Hong Xu, Jian Pei Fang, Wen Jun Weng, Hong Gui Xu. Critical Role of Sensitized Serum in Rejection of Allogeneic Bone Marrow Cells. Turk J Hematol. 2014; 31(3): 266-271

Corresponding Author: Lu Hong Xu, China


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